Antibiotic Resistance Gene Primers


LCPDb-ARG (Antibiotic Resistance Genes) is a subset of LCPDb - here we present primers extracted from published papers and analysed by our pipeline.
Original article available here

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Primers amplifying mphB gene


Ranking of available primer pair(s) :

#SpecificityEfficacyTaxonomy Efficacy
10.920.060.8
2000

WPS = Wrong Product Size

BOtG = Binds Outside the Gene

This heatmap shows if given primer pair (#) amplifies investigated gene variants from the pool of taxa in which all variants of particular gene were identified. (green = amplification, red = no amplification)

#1
#2
Bacillus
Escherichia
Streptococcus
Yersinia
uncultured
Product Length:

200 bp


Forward Primer Name:

mphB_F_(2)-353


Forward Primer Sequence (5' - 3'):

TGTGCCAGCAGGTACGATAG


Primer LengthPrimer GC%Primer Melting Temp.
20 bp55.00%53.83°C
Reverse Primer Name:

mphB_R_(2)-353


Reverse Primer Sequence (5' - 3'):

CCATACGCTGCTTCATTGAC


Primer LengthPrimer GC%Primer Melting Temp.
20 bp50.00%51.78°C
Specificity 92.00%

Efficacy 6.00%
Reference (primer origin)

Szczepanowski, Rafael et al. “Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.” Microbiology (Reading, England) vol. 155,Pt 7 (2009): 2306-2319. doi:10.1099/mic.0.028233-0