Antibiotic Resistance Gene Primers


LCPDb-ARG (Antibiotic Resistance Genes) is a subset of LCPDb - here we present primers extracted from published papers and analysed by our pipeline.
Original article available here

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Primers amplifying vgbA gene


Ranking of available primer pair(s) :

#SpecificityEfficacyTaxonomy Efficacy
1111

WPS = Wrong Product Size

BOtG = Binds Outside the Gene

This heatmap shows if given primer pair (#) amplifies investigated gene variants from the pool of taxa in which all variants of particular gene were identified. (green = amplification, red = no amplification)

#1
Plasmid_pIP630
Staphylococcus
Product Length:

188 bp


Forward Primer Name:

vgbA_F_(1)-365


Forward Primer Sequence (5' - 3'):

TCTGGGGGAAATTACCGAAT


Primer LengthPrimer GC%Primer Melting Temp.
20 bp45.00%49.73°C
Reverse Primer Name:

vgbA_R_(1)-365


Reverse Primer Sequence (5' - 3'):

AACAAATGCCATGTGGTTCA


Primer LengthPrimer GC%Primer Melting Temp.
20 bp40.00%47.68°C
Specificity 100.00%

Efficacy 100.00%
Reference (primer origin)

Szczepanowski, Rafael et al. “Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.” Microbiology (Reading, England) vol. 155,Pt 7 (2009): 2306-2319. doi:10.1099/mic.0.028233-0