LCPDb-ARG (Antibiotic Resistance Genes) is a subset of LCPDb - here we present primers extracted from published papers and analysed by our pipeline.
Original article available here
Ranking of available primer pair(s) :
| # | Specificity | Efficacy | Taxonomy Efficacy |
|---|---|---|---|
| 1 | 0.98 | 0.54 | 0.7 |
| 2 | 0.1 | 0.13 | 0.57 |
| 3 | 0.06 | 0.12 | 0.3 |
| 4 | 0 | 0.13 | 0.57 |
| 5 | 0 | 0 | 0 |
| 6 | 0 | 0 | 0 |
| 7 | 0 | 0 | 0 |
| 8 | 0 | 0 | 0 |
| 9 | 0 | 0 | 0 |
| 10 | 0 | 0 | 0 |
| 11 | 0 | 0 | 0 |
| 12 | 0 | 0 | 0 |
| 13 | 0 | 0 | 0 |
WPS = Wrong Product Size
BOtG = Binds Outside the Gene
This heatmap shows if given primer pair (#) amplifies investigated gene variants from the pool of taxa in which all variants of particular gene were identified. (green = amplification, red = no amplification)
471 bp
dfrA_F_(5)-461
AAAATTTCATTGATTTCTGCA
| Primer Length | Primer GC% | Primer Melting Temp. |
|---|---|---|
| 21 bp | 24.00% | 42.64°C |
dfrA_R_(5)-461
TTAGCCTTTTTTCCAAATCT
| Primer Length | Primer GC% | Primer Melting Temp. |
|---|---|---|
| 20 bp | 30.00% | 43.58°C |