Antibiotic Resistance Gene Primers


LCPDb-ARG (Antibiotic Resistance Genes) is a subset of LCPDb - here we present primers extracted from published papers and analysed by our pipeline.
Original article available here


Primers amplifying vanC-2 gene


Ranking of available primer pair(s) :

#SpecificityEfficacyTaxonomy Efficacy
10.970.711
20.910.51

WPS = Wrong Product Size

BOtG = Binds Outside the Gene

This heatmap shows if given primer pair (#) amplifies investigated gene variants from the pool of taxa in which all variants of particular gene were identified. (green = amplification, red = no amplification)

#1
#2
Enterococcus
Product Length:

439 bp


Forward Primer Name:

vanC-2_F_(1)-281


Forward Primer Sequence (5' - 3'):

CTCCTACGATTCTCTTG


Primer LengthPrimer GC%Primer Melting Temp.
17 bp47.00%44.64°C

Reverse Primer Name:

vanC-2_R_(1)-281


Reverse Primer Sequence (5' - 3'):

CGAGCAAGACCTTTAAG


Primer LengthPrimer GC%Primer Melting Temp.
17 bp47.00%44.64°C
Specificity 97.00%

Efficacy 71.00%