Antibiotic Resistance Gene Primers


LCPDb-ARG (Antibiotic Resistance Genes) is a subset of LCPDb - here we present primers extracted from published papers and analysed by our pipeline.
Original article available here

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Primers amplifying vatA gene


Ranking of available primer pair(s) :

#SpecificityEfficacyTaxonomy Efficacy
1111

WPS = Wrong Product Size

BOtG = Binds Outside the Gene

This heatmap shows if given primer pair (#) amplifies investigated gene variants from the pool of taxa in which all variants of particular gene were identified. (green = amplification, red = no amplification)

#1
Staphylococcus
Product Length:

268 bp


Forward Primer Name:

vatA_F_(1)-362


Forward Primer Sequence (5' - 3'):

TGGTCCCGGAACAACATTTAT


Primer LengthPrimer GC%Primer Melting Temp.
21 bp43.00%50.45°C
Reverse Primer Name:

vatA_R_(1)-362


Reverse Primer Sequence (5' - 3'):

TCCACCGACAATAGAATAGGG


Primer LengthPrimer GC%Primer Melting Temp.
21 bp48.00%52.4°C
Specificity 100.00%

Efficacy 100.00%
Reference (primer origin)

Safarpoor Dehkordi, Farhad et al. “Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food.” Antimicrobial resistance and infection control vol. 6 104. 4 Oct. 2017, doi:10.1186/s13756-017-0257-1